产品描述

General description

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser65, Gly69 and Tyr66).[5]

抗体与 GFP 融合蛋白发生特异性反应。

Application

利用小鼠体内产生的单克隆抗绿色荧光蛋白 (GFP) 抗体进行表面等离子体共振 (SPR) 金芯片分析，以研究半胱氨酸和结构域介导的增强型 GFP 模型蛋白的固定化。[1]

已成功使用该抗体的应用，相关同行评议论文如下。
• 免疫细胞化学
• 免疫荧光细胞培养细胞
• 免疫沉淀
• 免疫印迹
• 免疫沉淀后免疫印迹法

Biochem/physiol Actions

GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.[4]

绿色荧光蛋白产生于 维多利亚多管发光水母，可在能量转移反应中作为二级荧光蛋白产生绿光。它能够产生高度可见的内部荧光团，被广泛用作基因表达标记物，并能产生嵌合蛋白以了解蛋白质生物化学。[2][3]

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

Disclaimer

除非我们的产品目录或产品随附的其他公司文件中另有说明，否则我们的产品仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。